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primary mouse antibody against tuj1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary mouse antibody against tuj1
    Primary Mouse Antibody Against Tuj1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+mouse+antibody+against+tuj1/pm40007062-417-45-52?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    primary mouse antibody against tuj1 - by Bioz Stars, 2026-08
    86/100 stars

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    (a) RT-PCR analysis reveals differences in transcript levels for SOX9 and differentiation markers for neurons <t>(TuJ1),</t> astrocytes (GFAP) and oligodendrocytes (MBP) in the presence or absence of si SOX9 and/or NanoRU. (b) Quantitative comparison of the percentage of cells expressing <t>TuJ1</t> and GFAP. Student's unpaired t-test was used for evaluating the statistical significance for cells stained for TuJ1, compared to the si SOX9 on NanoRU condition (** = P < 0.001). (c) Fluorescence images of cells stained for the nucleus (blue), the neuronal marker TuJ1 (red, left column), the astrocyte marker GFAP (green, middle column) and merged (last column) show the extent of differentiation of NSCs grown on: no NanoRU nor si SOX9 coating (top row), NanoRU without si SOX9 coating (middle row), and NanoRU with si SOX9 coating (bottom row). Scale bars: 50 μm.
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    (a) RT-PCR analysis reveals differences in transcript levels for SOX9 and differentiation markers for neurons <t>(TuJ1),</t> astrocytes (GFAP) and oligodendrocytes (MBP) in the presence or absence of si SOX9 and/or NanoRU. (b) Quantitative comparison of the percentage of cells expressing <t>TuJ1</t> and GFAP. Student's unpaired t-test was used for evaluating the statistical significance for cells stained for TuJ1, compared to the si SOX9 on NanoRU condition (** = P < 0.001). (c) Fluorescence images of cells stained for the nucleus (blue), the neuronal marker TuJ1 (red, left column), the astrocyte marker GFAP (green, middle column) and merged (last column) show the extent of differentiation of NSCs grown on: no NanoRU nor si SOX9 coating (top row), NanoRU without si SOX9 coating (middle row), and NanoRU with si SOX9 coating (bottom row). Scale bars: 50 μm.
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    (a) RT-PCR analysis reveals differences in transcript levels for SOX9 and differentiation markers for neurons <t>(TuJ1),</t> astrocytes (GFAP) and oligodendrocytes (MBP) in the presence or absence of si SOX9 and/or NanoRU. (b) Quantitative comparison of the percentage of cells expressing <t>TuJ1</t> and GFAP. Student's unpaired t-test was used for evaluating the statistical significance for cells stained for TuJ1, compared to the si SOX9 on NanoRU condition (** = P < 0.001). (c) Fluorescence images of cells stained for the nucleus (blue), the neuronal marker TuJ1 (red, left column), the astrocyte marker GFAP (green, middle column) and merged (last column) show the extent of differentiation of NSCs grown on: no NanoRU nor si SOX9 coating (top row), NanoRU without si SOX9 coating (middle row), and NanoRU with si SOX9 coating (bottom row). Scale bars: 50 μm.
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    Image Search Results


    VM nondopaminergic neurons (TUJ1+/TH-) subjected to gabapentin exposure having no changes in the total neurite length (A) , the dominant neurite length (B) or the number of neurites (D) , while having significant increases in the number of branches (C) . Representative images and illustration for VM neurons immunolabeled with TUJ1 in both groups; control (E,E’) and gabapentin-treated (F,F’) show the increase in the numbers of branches in response to gabapentin exposure. (G,H) images show large field of view (20x) for both groups; control and gabapentin treated cultures, respectively. Scale bar = 50 um (E,F) . Scale bar = 10 um (G,H) . Data are represented as mean ± SEM, n = 4 experiments. * p < 0.05.

    Journal: Frontiers in Pharmacology

    Article Title: Prenatal Exposure to Gabapentin Alters the Development of Ventral Midbrain Dopaminergic Neurons

    doi: 10.3389/fphar.2022.923113

    Figure Lengend Snippet: VM nondopaminergic neurons (TUJ1+/TH-) subjected to gabapentin exposure having no changes in the total neurite length (A) , the dominant neurite length (B) or the number of neurites (D) , while having significant increases in the number of branches (C) . Representative images and illustration for VM neurons immunolabeled with TUJ1 in both groups; control (E,E’) and gabapentin-treated (F,F’) show the increase in the numbers of branches in response to gabapentin exposure. (G,H) images show large field of view (20x) for both groups; control and gabapentin treated cultures, respectively. Scale bar = 50 um (E,F) . Scale bar = 10 um (G,H) . Data are represented as mean ± SEM, n = 4 experiments. * p < 0.05.

    Article Snippet: The immunocytochemistry experiment were carried out using primary antibodies against mouse neuron-specific class III beta-tubulin (TUJ1) (Promega, G7121) and tyrosine hydroxylase (TH) (Abcam, ab112).

    Techniques: Immunolabeling, Control

    (a) RT-PCR analysis reveals differences in transcript levels for SOX9 and differentiation markers for neurons (TuJ1), astrocytes (GFAP) and oligodendrocytes (MBP) in the presence or absence of si SOX9 and/or NanoRU. (b) Quantitative comparison of the percentage of cells expressing TuJ1 and GFAP. Student's unpaired t-test was used for evaluating the statistical significance for cells stained for TuJ1, compared to the si SOX9 on NanoRU condition (** = P < 0.001). (c) Fluorescence images of cells stained for the nucleus (blue), the neuronal marker TuJ1 (red, left column), the astrocyte marker GFAP (green, middle column) and merged (last column) show the extent of differentiation of NSCs grown on: no NanoRU nor si SOX9 coating (top row), NanoRU without si SOX9 coating (middle row), and NanoRU with si SOX9 coating (bottom row). Scale bars: 50 μm.

    Journal: Scientific Reports

    Article Title: Nanotopography-mediated Reverse Uptake for siRNA Delivery into Neural Stem Cells to Enhance Neuronal Differentiation

    doi: 10.1038/srep01553

    Figure Lengend Snippet: (a) RT-PCR analysis reveals differences in transcript levels for SOX9 and differentiation markers for neurons (TuJ1), astrocytes (GFAP) and oligodendrocytes (MBP) in the presence or absence of si SOX9 and/or NanoRU. (b) Quantitative comparison of the percentage of cells expressing TuJ1 and GFAP. Student's unpaired t-test was used for evaluating the statistical significance for cells stained for TuJ1, compared to the si SOX9 on NanoRU condition (** = P < 0.001). (c) Fluorescence images of cells stained for the nucleus (blue), the neuronal marker TuJ1 (red, left column), the astrocyte marker GFAP (green, middle column) and merged (last column) show the extent of differentiation of NSCs grown on: no NanoRU nor si SOX9 coating (top row), NanoRU without si SOX9 coating (middle row), and NanoRU with si SOX9 coating (bottom row). Scale bars: 50 μm.

    Article Snippet: To study the extent of neuronal differentiation the primary mouse antibody against TuJ1 (1:500, Covance) and primary rabbit antibody against MAP2 (1:100, Cell Signaling) was used and for glial differentiation the primary rabbit antibody against GFAP (1:300, Dako) was used.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Comparison, Expressing, Staining, Fluorescence, Marker

    Primers used in PCR analysis

    Journal: Scientific Reports

    Article Title: Nanotopography-mediated Reverse Uptake for siRNA Delivery into Neural Stem Cells to Enhance Neuronal Differentiation

    doi: 10.1038/srep01553

    Figure Lengend Snippet: Primers used in PCR analysis

    Article Snippet: To study the extent of neuronal differentiation the primary mouse antibody against TuJ1 (1:500, Covance) and primary rabbit antibody against MAP2 (1:100, Cell Signaling) was used and for glial differentiation the primary rabbit antibody against GFAP (1:300, Dako) was used.

    Techniques: